ATP-stimulated breakdown of polylysine in rabbit reticulocyte cell-free extracts: differential effects according to substrate molecular weight
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چکیده
(a) 1 and 2: two successive extracts (30min each) from mouse liver chromatin; 3 and 4: the same from spleen chromatin; 5 and 6: the same from mouse kidney chromatin. Abbreviation used: LSP, liver specific protein. (b) 1-4: four successive 0.25 M-HCl extracts from mouse liver chromatin. Proteins were strained with Coomassie Brilliant Blue, and gel electrophoresis was performed by the method of Laemmli (1970). Chromatin was isolated from 30 mouse livers (35 g wet wt.). The first extraction yielded 40mg of total histones, the second extraction 4mg, the third extraction about 1 mg and the fourth extraction 0.61 mg of histones. TRANSACTIONS
منابع مشابه
ATP stimulates proteolysis in reticulocyte extracts by repressing an endogenous protease inhibitor.
An endogenous inhibitor of the reticulocyte ATP-dependent proteolytic system has been purified partially by ammonium sulfate precipitation from rabbit reticulocyte and erythrocyte extracts. Inhibitor-free protease rapidly degrades 21-40% of the substrate [14C]methyl-alpha-casein per hour, resembling ATP-dependent activity in reticulocyte extracts. This proteolytic activity is not stimulated by ...
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